Due to the potential impact in human health, the determination of the composition of Cannabis is of high importance. For this reason, different GC approaches have been carried out and were used to measure endocannabinoids, phytocannabinoids, synthetic cannabinoids, terpenes, among others. This technique requires previous derivatization of cannabinoids in order to measure their acidic form and increase their stability during the analysis. On the other hand, depending on the ionization mode, various features can be improved, such as selectivity, which can be enhanced working in the negative chemical ionization mode. GC coupled to different detectors is also employed for drug control in various biological samples, GC-MS being the most recurrent compared to FID or other detectors due to the quality of the results it provides. Furthermore, its versatility can be enhanced by changing the ionization source, thus improving sensitivity and/or selectivity of the measurements, as previously mentioned. On the other hand, including a previous extraction step, matrix effect, low concentration of the compounds as well as chromatographic resolution problems can be avoided. More sophisticated techniques have also been employed throughout the years for the determination of cannabinoids, namely GC-time-of-flight (TOF)/MS, however, these approaches are not easily accessible.